Six-way valve injector is a high performance liquid chromatography system, the ideal injector, which is sealed by a circular pad (rotor) and fixed-base (stator) component. The U.S. company's six-way Rheodyne valve injector of the most common, major manufacturers of HPLC instruments are this product as the instrument into the injector.
How it works:
1, handle-bit sample (Load) position, samples were micro-injection needle from the injection hole injection into the quantitative aspect of quantitative ring filled, the excess sample from the exhaust venting holes;
2, will rotate the handle to the injection (Inject) position, the valve connected with the liquid flow path from the pump delivered the mobile phase washed quantitative aspect of promoting the liquid sample into the analytical column for analysis.
Although the six-way valve injector with a simple structure, easy to use, long life, routine maintenance without the need characteristics, but the correct use and maintenance will be able to increase the service life and protect peripheral equipment, while increasing the analysis accuracy. If used properly, then the six-way valve injector generally continuous nebulization 30000 times without the need for maintenance.
The following six-way valve of the injector of the use and maintenance matters (for reference only)
1, the handle is between Load and Inject, due to a temporary blocking of the flow path, flow path in the pressure surge, and then go into the kind of place, high pressure caused damage to the stigma, it should be as soon as possible turn valves, can not be stay in the middle. In the HPLC system used syringe needles are different from gas chromatography, is a flat head injector. On the one hand, the outer needle injector sealing tube close to the inside, seal performance, no leakage, without introducing air; the other hand, it prevents a needle stab the bad seal components and stator.
2, the six-way valve into the sample injection device fitted methods are part of the solution method and full of liquid and French. France to use part of liquid into the sample, the sample size up to 75% of the volume of quantitative ring, such as the quantitative ring up 20gL sample 15p, L of the sample, and requires an accurate volume of each sample, the same; the use of fully loaded liquid sampling method when the sample size of at least quantitative ring size 3 to 5 times, that is, 20gL quantitative ring 60 to at least sample 1001aL samples, so as to completely replace the sample quantitative ring residual solution, to achieve the required precision degree and reproducibility. Recommended 100ul flat-head injection needle filled with 20ul sample loop.
3, injection volume according to the needs of their own making quantitative ring, generally does not require the accurate calculation of quantitative ring size, for example, a nominal 10gL quantitative ring, is actually 9gL or 1lgL not important, because the tested samples and calibration sample injection volume consistent errors in the calculation of the results have been offset.
4, injection sample particles and energy demands without resistance death of needles and injection valve material, the sample solution were to use 0.45 ~ tm of the membrane filter. To prevent particles blocking injection valve and reduce the wear and tear on injection valves. In order to prevent the buffer salts and other residual materials remain in the injection system, should be washed after each injection device, usually do not saline diluent, water or saline washing mobile phase, in the injection valve Load Inject position, and repeated washing, and then non-fiber paper to wipe the outside of syringe needles. ball valve Trunnion Mounted Ball Valve Floating Ball Valve Top Entry Ball Valve ball valve V Type Ball Valve
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